Quotation Kuntner, Claudia, Kesner, Adam L., Bauer, Martin, Kremslehner, Robert, Wanek, Thomas, Mandler, Markus, Karch, Rudolf, Stanek, Johann, Wolf, Tanja, Müller, Markus, Langer, Oliver. 2009. Limitations of Small Animal PET Imaging with [18F]FDDNP and FDG for Quantitative Studies in a Transgenic Mouse Model of Alzheimer’s Disease. Molecular Imaging and Biology 11 (4): 236-240.


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Abstract

Purpose We evaluated the usefulness of small animal brain positron emission tomography (PET) imaging with the amyloid-beta (Aβ) probe 2-(1-{6-[(2-[18F]fluoroethyl)(methyl)amino]-2-naphthyl}ethylidene)malonitrile ([18F]FDDNP) and with 2-deoxy-2-[F-18]fluoro-d-glucose (FDG) for detection and quantification of pathological changes occurring in a transgenic mouse model of Alzheimer’s disease (Tg2576 mice). Procedures [18F]FDDNP (n = 6) and FDG-PET scans (n = 3) were recorded in Tg2576 mice (age 13–15 months) and age-matched wild-type litter mates. Brain volumes of interest were defined by co-registration of PET images with a 3D MOBY digital mouse phantom. Regional [18F]FDDNP retention in mouse brain was quantified in terms of the relative distribution volume (DVR) using Logan’s graphical analysis with cerebellum as a reference region. Results Except for a lower maximum brain uptake of radioactivity in transgenic animals, the regional brain kinetics as well as DVR values of [18F]FDDNP appeared to be similar in both groups of animals. Also for FDG, regional radioactivity retention was almost identical in the brains of transgenic and control animals. Conclusions We could not detect regionally increased [18F]FDDNP binding and regionally decreased FDG binding in the brains of Tg2576 transgenic versus wild-type mice. However, small group differences in signal might have been masked by inter-animal variability. In addition, technical limitations of the applied method (partial volume effect, spatial resolution) for measurements in such small organs as mouse brain have to be taken into consideration.

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Publication's profile

Status of publication Published
Affiliation WU
Type of publication Journal article
Journal Molecular Imaging and Biology
Citation Index SCI
Language English
Title Limitations of Small Animal PET Imaging with [18F]FDDNP and FDG for Quantitative Studies in a Transgenic Mouse Model of Alzheimer’s Disease
Volume 11
Number 4
Year 2009
Page from 236
Page to 240
Reviewed? Y
URL http://www.springerlink.com/content/2500148t416k71j1/fulltext.pdf

Associations

People
Kremslehner, Robert (Former researcher)
External
Bauer, Martin (Department of Clinical Pharmacology, Medical University of Vienna, Vienna, Austria)
Karch, Rudolf (Department of Medical Computer Sciences, Medical University of Vienna, Vienna, Austria)
Kesner, Adam L. (Department of Molecular and Medical Pharmacology, UCLA, Los Angeles, CA, United States/USA)
Kuntner, Claudia (Department of Radiopharmaceuticals & microPET Imaging, Austrian Research Centers, Austria)
Langer, Oliver (Department of Radiopharmaceuticals & microPET Imaging, Austrian Research Centers, Austria)
Mandler, Markus (AFFiRiS GmbH, Vienna, Austria)
Müller, Markus (Department of Clinical Pharmacology, Medical University of Vienna, Vienna, Austria)
Stanek, Johann (Department of Radiopharmaceuticals & microPET Imaging, Austrian Research Centers, Austria)
Wanek, Thomas (Department of Radiopharmaceuticals & microPET Imaging, Austrian Research Centers, Austria)
Wolf, Tanja (Department of Toxicology, Austrian Research Centers, Austria)
Organization
Institute for Risk Management and Insurance IN (Former organization)
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